Author/Editor     Zagradišnik, Boris; Kokalj-Vokač, Nadja
Title     Hypomethylation of alphoid DNA and classical satellite DNA on chromosome 1, 9, 16 and Y in extraembryonic tissue
Type     članek
Source     Pflugers Arch
Vol. and No.     Letnik 440, št. 5 Suppl
Publication year     2000
Volume     str. R190-2
Language     eng
Abstract     The pattern of DNA methylation can he analyzed on methaphase chromosomes with fluorescein labeled antibodies against 5-methylcytosine. In human extmembryonic tissue lower overall intensity of immunofluorescence in centromeric chromosomal regions correspond to hypomethylation of the DNA when compared with normal human lymphocytes. Pericentromeric regions on chromosomes 1,9,16 and heterochromatin on chromosome Y, which reveal lower levels of immunofluorescence, are rich in classical satellite DNA type 2 and 3. In our experiment methylation-sensitive restriction enzymes, alphoid and classical satellite DNA probes specific for chromosomes 1,9,16 and Y were used. Southern blot analysis on cells from extraembryonic tissue revealed different extent of hypomethylation in different chromommal regions. Our results confirm overall and sequence-specific hypomethylation of DNA in cells from extraembryonic tissue in comparison with somatic cells.
Descriptors     CHORIONIC VILLI SAMPLING
DNA METHYLATION
DNA, SATELLITE
KARYOTYPING
DNA PROBES
LEUKOCYTES
CHROMOSOMES, HUMAN, PAIR 1
CHROMOSOMES, HUMAN, PAIR 9
CHROMOSOMES, HUMAN, PAIR 16
Y CHROMOSOME