| Author/Editor | Ferkolj, Ivan | |
| Title | Antineutrofilna citoplazmatska protutijela kod kroničnih upalnih crijevnih bolesti | |
| Type | monografija | |
| Place | Zagreb | |
| Publisher | Sveučilište u Zagrebu, Medicinski fakultet | |
| Publication year | 1997 | |
| Volume | str. 44 | |
| Language | cro | |
| Abstract | Ulcerative colitis and Crohn's disease belong to the group of inflammatory bowel diseases (IBD) of unknown etiology. In approximately 10% of IBD patients a definite distinction between two diseases cannot be made. IBDs are not usually classified as autoimmune disorders, but several factors indicate that immunological mechanisms may play a role in their pathogenesis. Several autoantibodies are associated with IBD, but anti- neutrophil cytoplasmic antibodies (ANCA), originally found to be associated with vasculitis, have been reported to be present in sera of a great number of patients with IBD, where most often the ANCA staining pattern is of the perinuclear type (pANCA). The aim of the study was to evaluate the prevalence of pANCA in our series of patients with IBD, the discriminatory value of these autoantibodies between UC and CD, their antigen specifity and their correlations with activity, extent and localisation of the disease, presence of extraintestinal disease manifestations and correlations with elevated values of ESR, CRP and platelet count. Serum anti-neutrophil cytoplasmic antibodies were evaluated by indirect immunofluorescence and by enzyme-linked immunosorbent assays (ELISA), using proteinase 3, myeloperoxidase, cathepsin G, lactoferrin, elastase and lysozime as antigens. pANCA were detected by immunofluorescence in 17 (71%) of 24 patients with ulcerative colitis and in 15 (31 %) of 48 patients with Crohn's disease (p<0,001). The predominant pattern was atypical perinuclear staining around neutrophil nuclei. ELISA gave no positive results and correlation of indirect immunofluorescence data and ELISA results indicate that even this large panel of specific antigens fails to identify target antigen. No significant correlation was found between the presence of pANCA and activity, localisation, extent, extraintestinal manifestations and eleveted laboratory parameters of inflammation (p>0,05). (Abstract truncated at 2000 characters.) | |
| Descriptors | INFLAMMATORY BOWEL DISEASES ANTIBODIES, ANTINEUTROPHIL CYTOPLASMIC PROCTOCOLITIS CROHN DISEASE COLITIS, ULCERATIVE FLUORESCENT ANTIBODY TECHNIQUE |