Author/Editor		Kralj, Jana; Naglič, Natalija; Gabrijelčič, Tone; Černelč, Peter
Title		Ocena aparata Coaguchek za hitro merjenje protrombinskega časa v kapilarni krvi
Translated title		Evaluation of Coaguchek instrument for prothrombin time determination in capillary blood
Type		članek
Source		Med Razgl
Vol. and No.		Letnik 39, št. Suppl 5
Publication year		2000
Volume		str. 55-61
Language		slo
Abstract		Background. Portable instruments that measure the prothrombin time (PT) and automatically calculate the international normalised ratio (INR) with the use of drop of whole blood have simplified the treatment of patients who are receiving warfarin therapy. We performed this study to determine the agreement between portable monitor and laboratory results. Methods. This study encompassed 133 patients (61 women and 72 men, aged 45 to years) on long-term anticoagulant therapy and 35 patients (19 women and 16 men, aged 55 to 80 years) starting anticoagulant therapy. Capillary blood samples were measured with CoaguChek (Boehringer Mannheim, Germany) instrument. Results were compared to those found with ACL-3000IL (Instrumentation laboratory) using citrated venous plasma. International sensitivity index of the thromboplastin used with ACL-3000 instrument was 1. 4. The difference in INR values between the two methods was calculated as the monitor value minus the clinical laboratory value. Agreement was achieved if the portable monitor and laboratory results were either within or outside the patients targeted therapeutie range or if the two results were within 0.4 international normalized ratio unites of each other. Results. The relationship between INR results from capillary blood samples measured with CoaguChek and venous plasma samples measured with ACL-3000 instrument in 133 patients on long-term anticoagulant therapy was expressed by r = 0,98, p < 0,001; y =1,04x +0,05 INR and in 35 patients starting anticoagulant therapy was expressed by r = 0,83, p < 0,05; y = 0,84x+0,61 INR. Agreement was observed in 97% patients on long-term anticoagulant therapy and in 54% patients starting anticoagulant therapy. (Abstract truncated at 2000 characters).
Summary		Izhodišča. Prenosni aparati za hitro merjenje protrombinskega časa (PČ) v kapilarni krvi, namenjeni samokontroli PČ bolnikov na domu, bi lahko zmanjšali število neprijetnih venepunkcij ter pogostih in rednih obiskov pri zdravniku in v laboratoriju. Zaradi nasprotujočih si ugotovitev o primerljivosti med izsledki določanja PČ v kapljici krvi in izsledki določanja PČ na uveljavljen način v krvni plazmi, smo se odločili primerjati merjenje PČ v kapilarni krvi na aparatu CoaguChek proizvajalca Boehringer Mannheim z merjenjem PČ v krvni plazmi na aparatu ACL-3000 proizvajalca Instrumentation Laboratory. Metode. Pri 133 bolnikih (61 žensk in 72 moških, starih od 45 do 75 let) z dolgotrajnim antikoagulacijskim zdravljenjem in pri 35 bolnik (19 žensk in 16 moških, starih od 55 do 80 let), ki so zdravljenje z varfarinom šele začeli, smo določili PČ na aparatu CoaguChek. Hkrati smo bolnikom določili PČ na tradicionalni način, z merjenjem časa strjevanja zmesi plazme bolnika, raztopine kalcijevega klorida in tkivnega tromboplastina. Meritve PČ smo napravili s analizatorjem ACL-3000. Pri delu smo uporabljali tovarniško pripravljene reagente in testne plazme proizvajalca Instrumentation Laboratory. Uporabili smo tromboplastin iz možganov kunca, test PT-HS (ISI = 1,47). Menili smo, da se meritve PČ v kapilarni krvi na aparatu CoaguChek in v krvni plazmi na aparatu ACL-3000 ujemajo, če sta bili obe meritvi znotraj ciljnega terapevtskega območja mednarodnega umeritvenega razmerja (INR), če sta bili obe meritvi nad ali pod terapevtskim območjem, če se obe meritvi med seboj nista razlikovali za več kot 0,4INR enote. Za oceno povezave med meritvami PČ na aparatu CoaguChek in na tradicionalen način smo uporabili analizo regresije in izračunali Spermanov korelacijski koeficient. Rezultati. Ugotovili smo, da so se v skupini 133 bolnikov z dolgotrajnim zdravljenjem z varfarinom pri 130 (97 %) meritve PČ na aparatu CoaguChek in na ACL-3000 ujemale. (Izvleček prekinjen pri 2000 znakih).
Descriptors		PROTHROMBIN TIME SELF-EXAMINATION ANTICOAGULANTS