Author/Editor		Piskar, Majda
Title		Določanje retikulocitnih parametrov na hematološkem analizatorju Coulter GEN S
Translated title		Evaluation of reticulocyte parameters on the Coulter GEN S hematology analyzer
Type		članek
Source		Med Razgl
Vol. and No.		Letnik 39, št. Suppl 7
Publication year		2000
Volume		str. 11-15
Language		slo
Abstract		For a long time the reticulocyte count has been used to estimate the erythropoetic activity of the bone marrow. An elevated reticulocyte number indicates increased erythropoiesis, which is normal response to various pathological conditions. After bone marrow damage or untreated anemias, decreased reticulocyte number indicated decreased erythropoiesis. The Hematology Laboratory at the Clinical Institute of Clinical Chemistry and Biochemistry, University Medical Centre, Ljubljana, introduced the flow cytometric method on the Coulter GEN S hematology analyzer, which greatly improved the precision and accuracy of reticulocyte counting. Reticulocytes have different amounts of RNA and they can be separated by their immaturity after staining and flow cytometric analysis. The immaturity level of a reticulocyte population can be determinated by a new additional reticulocyte parameters, such as the Immature Reticulocyte Fraction, which have already been proved as clinically and diagnostically useful.
Summary		Določanje števila retikulocitov se že dalj časa uporablja za oceno eritropoetske aktivnosti kostnega mozga. Zvečano število retikulocitov označuje povečano eritropoezo, ki je normalen odgovor na različna patološka stanja. Pri okvarah kostnega mozga ali pri anemijah, ki jih ne zdravimo, je število retikulocitov zmanjšano in pomeni zmanjšano eritropoezo. V hematološkem laboratoriju Kliničnega inštituta za klinično kemijo in biokemijo Kliničnega centra v Ljubljani smo na hematološkem analizatorju Coulter GEN S vpeljali metodo pretočne citometrije, ki je močno izboljšala ponovljivost in točnost štetja retikulocitov. Retikulociti vsebujejo različne količine RNA, zato se po obarvanju in analizi s pretočno citometrijo ločijo tudi po stopnji svojega dozorevanja. Za oceno dozorelosti se uporabljajo novi dodatni retikulocitni parametri, predvsem indeks nezrelih retikulocitov, ki so že dokazali svojo klinično in diagnostično uporabnost.
Descriptors		RETICULOCYTE COUNT ERYTHROCYTE COUNT ERYTHROCYTE INDICES FLOW CYTOMETRY METHYLENE BLUE