Author/Editor		Kopač, I; Batista, U
Title		Testiranje učinkov kemičnih retrakcijskih sredstev na celični kulturi fibroblastov
Translated title		Testing the effects of chemical retraction agents on fibroblast cell culture
Type		članek
Source		Zobozdrav Vestn
Vol. and No.		Letnik 55, št. 4-5
Publication year		2000
Volume		str. 110-7
Language		slo
Abstract		Chemical retraction agents used in fixed prosthodontics have more or less pronounced harmful effect on living cells. The present study using the dye exclusion test, colony forming ability test and colorimetric assay was undertaken to evaluate cytotoxic effects of four chemical retraction agents on cultured V-79 fibroblasts, and the dependence of cytotoxicity on the agent concentration and time of exposure. The most aggressive agent, 25 % aluminum chloride, took only one minute to damage all cell cultures. The proportion of cells damaged after 10 minutes of exposure to tetrahydrozoline was 60 %, which was significantly less compared to the other chemicals tested. With the colony forming ability test using retraction agents diluted to 1:10, the greatest number of colonies emerged in samples treated with tetrahydrozoline (statistical signifi- cance p < 0.01). The colorimetric assay showed equal cytotoxic effects for 25% aluminum sulphate and tetrahydrozoline. The colorimetric test used in the study has proved an ergonomic, accurate and reliable test for cytotoxicity determination.
Descriptors		FIBROBLASTS CELLS, CULTURED CELL SURVIVAL COLORIMETRY TETRAZOLIUM SALTS ALUMINUM COMPOUNDS CHLORIDES SULFATES TRYPAN BLUE DENTURE, PARTIAL, FIXED GINGIVA