Author/Editor		Fidler, N; Sauerwald, T; Demmelmair, H; Pohl, A; Koletzko, B
Title		Oxidation of an oil rich in docosahexaenoic acid compared to linoleic acid in lactating women
Type		članek
Source		Ann Nutr Metab
Vol. and No.		Letnik 43
Publication year		1999
Volume		str. 339-45
Language		eng
Abstract		Background: We studied the oxidation of an oil rich in docosahexaenoic acid (DHA; DHASCO) in lactating mothers receiving a dietary DHA supplement or a placebo. The results were compared with the oxidation of linoleic acid. Methods: Breast-feeding mothers received a dietary supplement (DHASCO; 200 mg DHA/day, n =5) or a placebo (n =5) for 14 days. Six weeks post partum all 10 mothers received a single dose of 2 mg/kg body weight uniformly 13C-labeled DHASCO. In a previously reported study 6 mothers received 1 mg/kg body weight uniformly 13C-labeled linoleic acid. Breath samples were collected over 48 h after tracer application. The total COz production was measured by indirect calorimetry and the 13C isotopic enrichment of labeled C02 by isotopic ratio mass spectrometry. Results: The oxidation of 13C-labeled DHASCO in the supplemented and placebo groups was similar. Maximal 13C enrichment was reached earlier in the group receiving 13C-DHASCO (median 1.0 vs. 3.0 h in the linoleic acid group). The cumulative 13C recovery in breath was higher in the DHASCO versus the linoleic acid group until 10 h after tracer application and comparable thereafter. Conclusions: The difference in oxidation of DHASCO versus linoleic acid after tracer ingestion might be partly due to a faster absorption and oxidation of shorter chain saturated fatty acids contained in DHASCO. The cumulative oxidation of DHASCO and linoleic acid 24 and 48 h aftertracer ingestion is similar.
Descriptors		LACTATION HEXANOIC ACIDS LINOLEIC ACIDS ADULT BREATH TESTS CARBON DIOXIDE DOUBLE-BLIND METHOD OXIDATION-REDUCTION