Author/Editor		Fidler, Nataša; Sauerwald, Thorsten; Pohl, Anja; Demmelmair, Hans; Koletzko, Berthold
Title		Docosahexaenoic acid transfer into human milk after dietary supplementation: a randomized clinical trial
Type		članek
Source		J Lipid Res
Vol. and No.		Letnik 41, št. 9
Publication year		2000
Volume		str. 1376-83
Language		eng
Abstract		Docosahexaenoic acid (DHA) is important for infant development. The DHA transfer from maternal diet into human milk has not been investigated in detail. We studied the effects of DHA supplementation on the fatty acid composition of human milk and the secretion of dietary 13C-labeled fatty acids, including DHA, into human milk. Ten lactating women were randomized to consume, from 4 to 6 weeks postpartum, an oil rich in DHA (DHASCO, 200 mg of DHA/day) (n=5) or a placebo oil (n=5). Dietary intakes were followed by 7-day protocols. On study day 14 a single dose of (U-13C)DHASCO was given orally, milk samples were collected over 48 h, and milk production was recorded. Milk fatty acid composition was determined by gas-liquid chromatography and isotonic enrichment was determined by gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). Milk DHA content did not differ between the supplemented and placebo group at study entry (0.29 vs. 0.28 wt%, median). After 2 weeks of supplememtation the milk DHA content was almost 2-fold higher in the supplemented was almost 2-fold higher in the supplemented versus placebo group (0.37 vs. 0.21 wt%, P=0.003). Cumulative recovery of (13C)palmitic, (13C)oleic, and (13C)docosahexaenoic acids in human milk at 48 h was similar between supplemented and placebo groups (palmitic acid 7.40 vs. 8.14%, oleic acid 9.14 vs. 9.97%, and docosahexaenoic acid 9.09 vs. 8.03% of dose, respectively). Notable lower recovery was observed for (13C)myristic acid in both the supplemented and placebo groups, 0.62 versus 0.77% of dose. Dietary DHA supplementation increases the DHA content in human milk. DHA transfer from the diet into human milk is comparable to palmitic and oleic acid transfer.
Descriptors		DIETARY FATS MILK, HUMAN HEXANOIC ACIDS FOOD LABELING LACTATION CHROMATOGRAPHY, GAS SPECTRUM ANALYSIS, MASS FATTY ACIDS, UNSATURATED CARBON ISOTOPES