Author/Editor		Fidler, Nataša
Title		Biologische Verfuegbarkeit der Docosahexaensaeure bei Stillenden Muettern
Translated title		Biološka razpoložljjivost dokozaheksaenojske kisline pri doječih materah
Type		monografija
Place		Ljubljana
Publisher		Biotechnische Fakultaet, Abteilung fuer Lebensmitteltechnologie
Publication year		1999
Volume		str. 109
Language		ger
Abstract		We aimed at investigating the transfer of DHA and other fatty acids from the diet into the human milk and plasma lipid fractions, as well as the course of oxidation of the nutrition supplement rich in DHA. Starting four weeks post partum, ten lactating women consumed for 13 days either 200 mg DHA/day (n=5) either a placebo (n=5). On the study day 14 all mothers consumed 2 mg 13C labelled DHASCO TM/kg body weight. Samples of milk, blood and breath were collected before and during 48 hours after the tracer ingestion. Fatty acid composition of milk and plasma lipids was determined by GC and the 13C isotopic enrichments of milk fatty acids and breath air by GC-C-IRMS. After the DHA supplementation the DHA content of milk lipids from the supplemented group was higher than from the placebo group (0.37 vs 0.21 wt.%, median, p=0.003). Cumulative recovery of dietary 13C labelled myristic, palmitic, oleic acid and DHA in human milk at 48h was similar in both groups 0.8, 7.8, 9.6, and 8.0%. The DHA content in plasma phospholipids of women from the supplemented vs. placebo group was 3.25 vs. 2.33 wt.% (p=0.004) and in the plasma-triglycerides 0.79 vs. 0.29 wt.% (p=0.003). Cumulative oxidation of 13C-DHASCO TM was similar in the supplemented and the placebo group (14.2 vs. 15.6%). We conclude that maternal dietary intake of DHA is a major determinant of its content in milk and plasma lipids. About 20% of the dietary supplemented DHA over the two week period is secreted into human milk. The transfer of DHA from the diet into human milk is comparable to that of palmitic and oleic acids. Remarkable lower transfer of myristic acid into human milk could possibly be due to its higher oxidation rate.
Descriptors		BREAST FEEDING LACTATION MILK, HUMAN DIETARY FATS DOCOSAHEXAENOIC ACIDS PREGNANCY CHROMATOGRAPHY, GAS SPECTRUM ANALYSIS, MASS TRIGLYCERIDES