Author/Editor		Cerar, Anton; Poljak, Mario; Volavšek, Metka
Title		Human pailloma virus infection and p53 protein expression in squamous cell papilloma of the oesophagus
Type		članek
Source		In: Gale N, Žargi M, editors. Epithelial tumours of the head and neck. Proceedings of the 31st memorial meeting for professor Janez Plečnik under the auspices of the European society of pathology; 2000 Dec 7-8; Ljubljana. Ljubljana: Faculty of medicine, Institute of pathology,
Publication year		2000
Volume		str. 125-7
Language		eng
Abstract		The aetiology and pathogenesis of oesophageal squamous cell papilloma (OSCP), a rare tumor of oesophagus, are still controversal. Two proposed etiologies are chronic oesophageal irritation and infection with human papillomaviruses (HPV). On the other side, it is not clear if OSCP represents a premalignant lesion. In the present study, 36 cases of OSCP we screened for HPV infection using in situ hybridisation (ISH) and polymerase chain reaction (PCR). Additionally, p53 immunoreactivity using the monoclonl antibody PAb 1801, was determined. The PCR for HPV type 6 followed by restriction fragment analysis of the PCR product, showed positivity in two cases of OSCP. No HPV DNA was found using ISH. p53 showed immunoreactivity in five cases. In two cases positive epithelial cells were found only very occasionally.In additional three OSCPs, p53 staining was restricted to the basal epithelial cells. Both HPV-positive OSCPs were p53 negative: We suppose that our findings represent immunohistochemical detection of the stabilized wild-type p53 protein rather than mutated p53 protein, and.supporr low malignant potential of OSCP: Our results show that HPV is rarely detectable in OSCPs and that other pathogenetic mechanisms are more important in aetiology of OSCP. The findinig of p53 overexpression in relatively low number of cases, finding of "low risk" HPV types 6 and 11, and the absence of epithelial dysplasia in OSCP suggest that OSCP has no malignant potential.
Descriptors		ESOPHAGEAL NEOPLASMS CARCINOMA, SQUAMOUS CELL PROTEIN P53 PAPILLOMAVIRUS, HUMAN IN SITU HYBRIDIZATION POLYMERASE CHAIN REACTION BIOPSY