Author/Editor		Coer, Andrej; Luzar, Boštjan; Zidar, Nina; Gale, Nina
Title		Cell cycle regulation proteins p21WAF1/CIP1 and p27KIP1 in squamous cell carcinoma of the larynx
Type		članek
Source		In: Gale N, Žargi M, editors. Epithelial tumours of the head and neck. Proceedings of the 31st memorial meeting for professor Janez Plečnik under the auspices of the European society of pathology; 2000 Dec 7-8; Ljubljana. Ljubljana: Faculty of medicine, Institute of pathology,
Publication year		2000
Volume		str. 149-52
Language		eng
Abstract		One of the main properties of cancer cells is their increased and deregulated proliferative activity. p21WAF1/CIP1 and p2Kip1 are members of the CIP/Kip family of cyclin dependent kinase inhibitor (CKI) proteins that negatively regulate cell proliferation. To improve our understanding of the role and clinical relevance of p21WAF1/CIP1 and p2Kip1 in squamous cell carcinoma (SCC) of the larynx, we analysed by immunohistochemistry p21WAF/CIP1 and p2Kip1 expression in a series of 30 patients who had undergone laryngectomy due to the laryngeal SCC. Proliferative activity was assessed in serial section by determining the expression of the cell cycle protein Ki-67. We found a statistically significant correlation between the grade of SCC and the percentage of Ki-67 positive cells (P<0.05). p21WAF1/CIP1 expression was strongly associated with squamous cell differentiation of carcinoma, because all seven carcinomas grade III showed very low levels of p21WAF1/CIP1 expression. No significant correlation between p21WAF1/CIP1 and Ki-67 was found. p27Kip protein was significantly reduced in carcinomas compared with that in normal epithelial controls. There was a negative correlation between the Ki-67 labelling index and p27Kip1 expression. In conclusion, this study has found that a reduction in the proportion of cells expressing p21WAF1/CIP1 and p27Kip1 protein is frequently associated with SCC of the larynx.
Descriptors		LARYNGEAL NEOPLASMS CARCINOMA, SQUAMOUS CELL CELL CYCLE PROTEINS