Author/Editor		Abram, Maja; Vučković, Darinka; Wraber, Branka; Dorić, Miljenko
Title		Plasma cytokine response in mice with bacterial infection
Type		članek
Source		Mediat Inflamm
Vol. and No.		Letnik 9
Publication year		2000
Volume		str. 229-34
Language		eng
Abstract		Background: Exposure to microorganisms elicts the production of cytokines. These soluble factors enhance several innate immune functions and regulate the ensuing specific immune response aimed at limiting the spread of infection. Aim: This study was undertaken to quantify the plasma levels of pro-inflammatory cytokines during the course of primary Listeria monocytogenes and Campylobacter jejuni infection. Using an in vivo infection the relationship between endogenous cytokines and the bacterial number in the liver of infected animals was examined. Methods: C5713L/6 mice were infected by the intraperitoneal route. At different time points we determined the number of colony-forming units of bacteria in the liver of infected animals and paralled these with the plasma levels of interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha) and interleukin6 (IL-6) measured by enzyme immunoassays. Results: L. monocytogenes infection lasted 10-11 days. IFN-gamma production occurred in the early phase but was more pronounced after day 4, following the appearance of specific immunity. The duration of experimental campylobacteriosis was 15 days. Early IFN-gamma production was not significant but a progressive rise of this cytokine in plasma was seen during the second week post infection. (Abstract truncatet at 2000 characters)
Descriptors		CAMPYLOBACTER JEJUNI INTERFERON TYPE II TUMOR NECROSIS FACTOR INTERLEUKIN-6 LISTERIA MONOCYTOGENES MICE, INBRED C57BL DISEASE MODELS, ANIMAL LIVER