Author/Editor		Petrovič, D; Zorc, M; Peterlin, B
Title		Effect of apolipoprotein E polymorphism and apolipoprotein A-1 gene promoter polymorphism on lipid parameters and premature coronary artery disease
Type		članek
Source		Folia Biol (Praha)
Vol. and No.		Letnik 46
Publication year		2000
Volume		str. 181-5
Language		eng
Abstract		Genetic and environmental factors regulate lipid metabolism and phenotypic expression of CAD. In this study we assessed the effects of apoE gene polymorphism and apo A1 gene promoter polymorphism on lipid metabolism and risk for CAD. In a case-control study, 166 patients with CAD were compared with 130 healthy subjects. The apoE allele frequencies of paticnts vs. control group were 6.3% vs. 7.7% for e2, 84.3% vs. 84.6 % for e3, and 9.4 % vs. 7.7% for e4. Individuals with e3e4 and e4e4 genotypes had higher total (P=0.023) and LDL cholesterol levels (P=0.04) than individuals with other genotypes. There were no differences in lipid parameters between the subjects with the apoAl-GG genotype and subjects with AG or AA genotypes. However, univariate analysis revealed no association betwcen risk genotypes (e3e4 and e4e4 genotypes) of apoE and CAD risk (OR=1.1; 95% CI=0.6-2.1, P=0.8) as well as no association between the GG genotype and CAD risk (OR 0.7; 95 % CI=0.5-1.2, P=0.19). No evidence for a synergistic interaction between e3c4 plus e4e4 genotypes and apoAl-GG genofype on CAD risk was fonnd (OR = 1.3, 95% CI=0.6-2.9; P=0.5). One individual with familial defective apolipoprotein B-100 (Arg3500G1n) was found in cach group. In conclusion, the apoE gene polymorphism affected the total and LDL cholesterol levels, whereas neither the apoE gene polymorphism nor the apoA-1 gene promoter polymorphism were shown to be independent risk factors for CAD in Slovenia.
Descriptors		CORONARY DISEASE APOLIPOPROTEINS E POLYMORPHISM (GENETICS) APOLIPOPROTEIN A-I CASE-CONTROL STUDIES LIPOPROTEINS, LDL CHOLESTEROL LIPOPROTEINS, HDL CHOLESTEROL GENOTYPE POLYMERASE CHAIN REACTION CHOLESTEROL TRIGLYCERIDES