Author/Editor     Trontelj, Katja; Kandušer, Maša; Miklavčič, Damijan
Title     Influence of vinblastine on electroporation of B16F1 cell line
Type     članek
Source     In: Jarm T, Kotnik T, Miklavčič D, editors. Proceedings including program of the 3rd international conference on bioelectromagnetism and 1st Slovenian-Croatian meeting on biomedical engineering in connection with COST 244bis 11th workshop on biomedical applications of electromagnetic fields and management commitee meeting; 2000 oct 8-12; Bled. Ljubljana: Faculty of electrical engineering,
Publication year     2000
Volume     str. 29-30
Language     eng
Abstract     Among parameters affecting cell electroporation is also the integrity of cytoskeleton. This preliminary study focuses on changes in electroporation due to vinca alkaloid vinblastine that interacts with cytoskeleton. Permeabilization was determined with bleomycin uptake assay and irreversible electroporation was determined with cell survival test. B16F1 cell line was used in experiments. We applied electrical parameters that are usual in electrochemotherapy: 8 pulses of 100 micro s duration at 1 Hz and voltages in a range between 0 (control) and 400 V. We used nontoxic concentration of vinblastine. Vinblastine did not affect neither the reversible nor the irreversible electroporation at our concentration, parameters and time of incubation with vinblastine.
Descriptors     MELANOMA, EXPERIMENTAL
VINBLASTINE
ELECTROPORATION
DRUG DELIVERY SYSTEMS
MICE
CYTOSKELETON
CELL SURVIVAL