Author/Editor     Pucihar, Gorazd; Miklavčič, Damijan
Title     The influence of conductivity of extracellular media on permeabilization and survival of the cells after electroporation
Type     članek
Source     In: Jarm T, Kotnik T, Miklavčič D, editors. Proceedings including program of the 3rd international conference on bioelectromagnetism and 1st Slovenian-Croatian meeting on biomedical engineering in connection with COST 244bis 11th workshop on biomedical applications of electromagnetic fields and management commitee meeting; 2000 oct 8-12; Bled. Ljubljana: Faculty of electrical engineering,
Publication year     2000
Volume     str. 31-2
Language     eng
Abstract     Induced transmembrane voltage depends among other things on extracellular, intracellular and membrane conductivity. Since extracellular conductivity is easy to manipulate with, we decided to take three media with different conductivities and study how decreasing the extracellular conductivity influence the induced transmembrane voltage. This was studied by comparing the survival and permeabilization of the cells in these media. Comparing experimental and calculated results, showed good agreement in survival of the cells, on the contrary medium conductivity had no effect on permeabilization results.
Descriptors     FIBROBLASTS
CELL MEMBRANE PERMEABILITY
ELECTROPORATION
EXTRACELLULAR SPACE
ELECTRIC CONDUCTIVITY
CELL SURVIVAL
MEMBRANE POTENTIALS