Author/Editor		Bernard, Rok; Kandušer, Maša; Pernuš, Franjo
Title		Evaluation of an automated system for cell colony counting
Type		članek
Source		In: Jarm T, Kotnik T, Miklavčič D, editors. Proceedings including program of the 3rd international conference on bioelectromagnetism and 1st Slovenian-Croatian meeting on biomedical engineering in connection with COST 244bis 11th workshop on biomedical applications of electromagnetic fields and management commitee meeting; 2000 oct 8-12; Bled. Ljubljana: Faculty of electrical engineering,
Publication year		2000
Volume		str. 85-6
Language		eng
Abstract		The performance of an automated cell colony counting system, based on computer vision technology, is evaluated. Cell colonies were formed from DC-3F cells, fixed with methanol and stained with crystal violet. The accuracy of automated cell colony detection was measured on 17 petri dishes. The system is in average able to correctly detect 91% of cell colonies on medium quality images. The cell colonies of various sizes and intensities are reliably detected even in case they overlap or form clusters. Next the correlation with manual counting method is examined. 60 petri dishes were analysed and the correlation coefficient of 0.995 was obtained. The comparison of survival rate curves obtained by automated and manual methods show the variation of the cell colony formation process is larger than the variability between both methods.
Descriptors		CELLS, CULTURED CELL COUNT IMAGE PROCESSING, COMPUTER-ASSISTED CELL SURVIVAL CELL SIZE