Author/Editor		Mlinarič, Aleš; Kreft, Samo; Umek, Andrej; Štrukelj, Borut; Popovič, Tatjana
Title		Cysteine proteinase inhibitors screening of fungal species growing in Slovenia
Translated title		Iskanje inhibitorjev cisteinskih proteinaz v glivah s področja Slovenije
Type		članek
Source		Acta Pharm Zagreb
Vol. and No.		Letnik 50, št. 1
Publication year		2000
Volume		str. 39-48
Language		eng
Abstract		Ninety extracts obtained from fungi growing in Slovenia were screened for cysteine proteinase inhibitory activity using in vitro assay. Papain, human cathepsin B and human cathepsin H were the three enzymes used in this study. Our results suggest that at least two fungi from all the samples examined might contain substances with inhibitory activity. One fraction of the extract obtained from Cortinarius violaceus (L. Fr.) Fr. (Basidiomycotina) and two fractions of the extract obtained from Gloeophyllum odoratum (Wulf. Fr.) Imaz. (Basidiomycotina) exhibited the highest inhibitory activity and were selected for further investigation.
Summary		Izvlečkom iz devetdesetih vrst gliv s področja Slovenije smo v okviru naše raziskave s pomočjo in vitro testnega sistema preizkusili inhibitorno aktivnost na cisteinske proteinaze. Izbrane proteinaze so bile papain, humani katepsin B in humani katepsin H. Ugotovili smo, da bi vsaj dve glivi lahko vsebovali učinkovine z inhibitornim delovanjem. Najvišjo inhibitorno aktivnost smo zasledili pri eni frakciji izvlečka glive Cortinarius violaceus (L. Fr.) Fr. (Basidiomycotina) in pri dveh frakcijah izvlečka glive Gloeophyllum odoratum (Wulf. Fr.) Imaz. (Basidiomycotina). Omenjene frakcije so predmet nadaljnih raziskav.
Descriptors		BASIDIOMYCETES CYSTEINE PROTEINASE INHIBITORS ASCOMYCETES SLOVENIA CATHEPSINS PAPAIN CHROMATOGRAPHY