Author/Editor		Podlesek, Zdravko; Comino, Aleksandra; Herzog-Velikonja, Blagajana; Grabnar, Miklavž
Title		The role of the bacitracin ABC transporter in bacitracin resistance and collateral detergent sensitivity
Type		članek
Source		Fems Microbiol Lett
Vol. and No.		Letnik 188
Publication year		2000
Volume		str. 103-6
Language		eng
Abstract		The bacitracin resistance of Bacillus licheniformis, a producer of bacitracin, is mediated by the ABC transporter Bcr. Bacillus subtilis cells carrying bcr genes on high-copy number plasmids developed collateral detergent sensitivity, as did human cells with overexpressed multidrug resistance P-glycoprotein. Resistance against bacitracin and sensitivity of resistant cells to detergents were shown to be inseparable phenomena associated with the membrane part of Bcr transporter, namely protein BcrC. A fused protein, consisting of ATP-binding protein BcrA and: membrane component BcrC was constructed, It resembled a half molecule of P-glycoprotein and was capable of providing a significant degree of antibiotic resistance and detergent sensitivity.
Descriptors		BACILLUS BACITRACIN DRUG RESISTANCE, MICROBIAL ABC TRANSPORTERS P-GLYCOPROTEIN DETERGENTS BACILLUS SUBTILIS ESCHERICHIA COLI TRANSFORMATION, GENETIC PROTOPLASTS