| Author/Editor | Kos, Janko; Krašovec, Marta; Cimerman, Nina; Nielsen, Hans Jorgen; Christense, Ib Jarle; Bruenner, Nils | |
| Title | Cysteine proteinase inhibitors stefin A, stefin B, and cystatin C in sera from patients with colorectal cancer: relation to prognosis | |
| Type | članek | |
| Source | Clin Cancer Res | |
| Vol. and No. | Letnik 6 | |
| Publication year | 2000 | |
| Volume | str. 505-11 | |
| Language | eng | |
| Abstract | The levels of cysteine proteinase inhibitors stefin A, stefin B, and cystatin C were determined using ELISAs in sera obtained preoperatively from 345 patients with colorectal cancer and in control sera from 125 healthy blood donors. The levels of stefin A and cystatin C were found to be moderately increased in patient sera (1.4-fold and 1.6 fold, respectively; P < 0.0001), whereas the level of stefin B remained statistically unchanged when compared with controls. The medians were 4.3 ng/ml versus 3.2 ng/ml for stefin A, 1.2 ng/ml versus 1.7 ng/ml for stefin B, and 679 ng/ml versus 425 ng/ml for cystatin C. In patient sera, a weak correlation of cystatin C with age (r =0.34; P < 0.001) and gender (P=0.01) was found. Stefin A and cystatin C levels were independent of Dukes' stage, whereas stefin B correlated significantly with Dukes' stage, its level being the highest in stage D (P < 0.007). Stefin B and cystatin C correlated with survival, whereas stefin A was not a significant prognostic factor in this study. Using medians as cutoff values, patients with high levels of stefin B and patients with high levels of cystatin C exhibited a significantly higher risk of death than those with low levels of inhibitors (hazard ratio =1.6; 95% confidence interval,1.2-2.2; P=0.002 for stefin B; hazard ratio = 1.3; 95% confidence interval, 1.01.8; P=0.04 for cystatin C). Our results reveal a correlation between high levels of extracellular cysteine proteinase inhibitors and short survival in patients with colorectal cancer and the data thus support previous studies suggestion a contributing role of protease inhibitors in the progression of cancer. | |
| Descriptors | CYSTEINE PROTEINASE INHIBITORS COLORECTAL NEOPLASMS ENZYME-LINKED IMMUNOSORBENT ASSAY PROGNOSIS CYSTATINS SURVIVAL ANALYSIS MULTIVARIATE ANALYSIS |