Author/Editor | Werle, Bernd; Kraft, Clemens; Lah, Tamara T; Kos, Janko; Schanzenbaecher, Ulrike; Kayser, Klaus; Ebert, Werner; Spiess, Eberhard | |
Title | Cathepsin B in infiltrated lymph nosed is of prognostic significance for patients with nonsmall cell lung carcinoma | |
Type | članek | |
Source | Cancer | |
Vol. and No. | Letnik 89, št. 11 | |
Publication year | 2000 | |
Volume | str. 2282-91 | |
Language | eng | |
Abstract | Background.Tumor cells require specific proteolytic enzymes for invasion and metastasis, including lysosomal peptidases-cathepsins. Cathepsin B is a lysosomal cysteine peptidase, which appears to play a major role in invasion and metastasis of human tumors. In this study, the authors focused on the possible role of cathepsin B in lymphogenic metastasis by investigating the enzyme localization and its activity in lung tumors,and corresponding tumor-infiltrated lymph nodes. Methods. Cathepsin B activity was determined in lung tumors, lung parenchyma, and tumor cell-infiltrated and noninfiltrated regional lymph nodes of the same patient. The authors investigated 35 cancer patients suffering from nonsmall cell lung carcinoma. Cathepsin B throughout activity was measured by cleavage of the fiuorogenic substrate Z-Arg-Arg-AMC at pH 6.0. Results. The median specific cathepsin B activity was highest in tumors, followed by the infiltrated lymph nodes, noninfiltrated lymph nodes, and lung parenchyma. The authors showed a significant 1.8-fold increase in cathepsin B activity in tumor-infiltrated lymph nodes compared with noninfiltrated regional lymph nodes and a 4.5-fold increase in lung tumor tissue compared with lung parenchyma. High cathepsin B activity, both in tumors and tumor cell-infiltrated lymph nodes, indicated poor prognosis for overall survival. Immunohistochemical analysis showed the presence of cathepsin B in histiocytes and tumor cells but not in lymphocytes of lymph node tissue. Conclusions. The authors' findings on higher cathepsin B levels in tumor cellinfittrated lymph nodes show that increased level of cathepsin B activity is characteristic of the invasive tumor cell phenotype. This corroborates the hypothesis, that tumor cell associated cathepsin B may play a role in lymphogenic metastasis. The authors' results support the use of lymph node associated cathepsin B as a prognostic factor for survival of patients with lung carcinoma. | |
Descriptors | CARCINOMA, NON-SMALL-CELL LUNG LYMPHATIC METASTASIS CATHEPSIN B PROGNOSIS SURVIVAL ANALYSIS ADENOCARCINOMA CARCINOMA, SQUAMOUS CELL LYMPH NODE EXCISION NEOPLASM STAGING IMMUNOHISTOCHEMISTRY LUNG NEOPLASMS |