Author/Editor     Fležar, M; Doudkine, A; Us-Krašovec, M
Title     The effect of primary fixation with standard postfixation and the duration of hydrolysis on nuclear features in image cytometry
Type     članek
Source     Anal Cell Pathol
Vol. and No.     Letnik 17, št. 3
Publication year     1998
Volume     str. 131-44
ISSN     0921-8912
Language     eng
Abstract     The effect of three primary fixation procedures, used in the preparation of routine cytological samples: air-drying, Delaunay, and Saccomanno fixation, with postfixation in modified Bohm-Sprenger fixative, on nuclear features as a function of hydrolysis time is reported. Three different cell types: lymphatic cells (tonsil), epithelial cells (buccal mucosa) and mesenchymal cells (uterine myometrium) were used for the study. Our findings show, that generally not all features have the same plateau times as the IOD (integrated optical density), and that many features show different values depending on cell type and fixation method. It is therefore recommended that for any primary fixative used in routine clinical work and for each cell type, the hydrolysis curve for all nuclear features to be used in sample analysis should be established.
Descriptors     TISSUE FIXATION
IMAGE CYTOMETRY
ANALYSIS OF VARIANCE
FIXATIVES
MARKOV CHAINS
MOUTH MUCOSA
TIME FACTORS
TONSIL
UTERUS