Author/Editor		Baumann, Frank; Milisav, Irina; Neupert, Walter; Herrmann, Johannes M
Title		Ecm 10, a novel Hsp70 homolog in the mitochondrial matrix of the yeast Saccharomyces cerevisae
Type		članek
Source		Febs Lett
Vol. and No.		Letnik 487
Publication year		2000
Volume		str. 307-12
Language		eng
Abstract		Members of the heat shock protein 70 (Hsp70) family are found in most of the compartments of eukaryotic cells where they play essential roles in protein metabolism. In yeast mitochondria, two Hsp70 proteins are known: Sscl and Ssql. We identified Ecm10 as a third Hsp70 protein in the mitochondrial matrix. Ecm10 shares 82% amino acid identity with Sscl and 54% with Ssq1. Overexpression of Ecm10 mitigates protein import defects in sscl mutants suggesting that Ecm10 can play a role in protein translocation. Like Sscl, Ecm10 interacts with the nucleotide exchange factor Mgel in an ATP-dependent manner. Deletion of ecm10 leads to synthetic growth defects with sscl mutations at low temperature. Our data suggest an overlapping function of Ecm10 and Ssc1.
Descriptors		SACCHAROMYCES CEREVISIAE HEAT-SHOCK PROTEINS 70 MITOCHONDRIA MOLECULAR CHAPERONES TRANSCRIPTION, GENETIC TRANSLATION, GENETIC POLYMERASE CHAIN REACTION BASE SEQUENCE EPITOPES