Author/Editor		Šabovič, M; Keber, D
Title		Factors influencing the lysis of ex vivo human thrombi
Type		članek
Source		Fibrinolysis
Vol. and No.		Letnik 10, št. 2
Publication year		1996
Volume		str. 103-9
Language		eng
Abstract		The lysis of human ex vivo thrombi was studied in vitro in relation to their age, the thrombolytic agent (streptokinase, urokinase or t-PA), the thrombolytic milieu (plasma or buffer) and the addition of lys-plasminogen (lys- plg). Fourteen arteriai and 16 venous thrombi were obtained at autopsies or operations. The probable thrombi age was estimated by the time interval from the onset of clinical symptoms and in vivo imaging to removai. Pieces of the thrombi, weighing approximately 250 mg, weče exposed to lysis in 2 ml volumes of plasma or buffer; and the extent of lysis was measured by the decrease of the wet weight of thrombi over a period of 24 h. A nonsignificantly better lysis (from 5-15% with different agents) was found in fresh arterial and venous thrombi (age of less than 1 week, median value 2 days) in comparison to aged thrombi (older than 1 month, median value 9 weeks). All three thrombolytic agents were equally effective towards fresh and aged thrombi. The substitution of consumed plasminogen by lys-plg improved the lysis; excellent improvement was obtained when intermittent addition of activator and lys-plg was applied. Finally, we found that lysis of ex vivo thrombi in buffer milieu significantly exceeded that in plasma milieu. In conclusion, the general belief in the decrease of thrombus lysability during the course of several months ageing was not confirmed by this study. The lysis of ex vivo thrombi could be substantially improved by supplementing consumed plasminogen or by replacing lytic mitieu from plasma to buffer. These findings in ex vivo thrombi might have potential value for the improvement of clinical thrombolysis.
Descriptors		CLOT RETRACTION FIBRINOLYTIC AGENTS STREPTOKINASE UROKINASE ALTEPLASE PLASMINOGEN DOSE-RESPONSE RELATIONSHIP, DRUG