| Author/Editor | Kotnik, Ana | |
| Title | Splenektomija in imunski odziv | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Medicinska fakulteta | |
| Publication year | 2000 | |
| Volume | str. 36 | |
| Language | slo | |
| Abstract | Backgrounds. Removal of the spleen reduces resistance of the patient to infections, most frequently those caused by pneumococci. The most effective way of protecting these patients is vaccination with a 23-valent pneumococcal vaccine. The aim of our research učas to establish at what time after splenectomy vaccination is most effective. Different possibilities were studied: vaccination just before splenectomy, vaccination one day after and seven days after splenectomy. Our hypothesis was that vaccination just before splenectomy has the best protective effect from infection. Methods. The prospective study was carried out on thirty mice divided into six groups. Their spleens were surgically removed and they were vaccinated at different times before or after the operation. The first group was vaccinated, without splenectomy. The second group was vaccinated just before splenectomy, the third group 1 day after and the fourth group 7 days after surgery. The fifth group had their spleens removed without vaccination. The sixth group consisted of healthy, unvaccinated mice. During the study, the mice had their blood drawn four times. With an enzyme-linked immunosorbent assay, the changing of the amount of specific IgM and IgG antibodies directed against pneumococcal antigens was measured. The amount of precipitating antibodies in the blood serum of mice was measured with a microprecipitation capillary test. The study was concluded with a supervised infection of mice with live pneumococci. Results. The amount of IgG, IgM and precipitating antibodies was at all times highest in the group of immunized unsplenectomiced mice (group 1). A statistically significant difference was found between this and all other groups (p,0.05). Between the splenectomized groups of mice, mice immunized 1 day after splenectomy (group 3) produced the highest amount of specific antibodies against Streptococcus pneumoniae. (Abstract truncated at 2000 characters). | |
| Descriptors | SPLENECTOMY IMMUNITY MICE PROSPECTIVE STUDIES VACCINES ANTIBODIES SPLEEN STREPTOCOCCUS PNEUMONIAE ENZYME-LINKED IMMUNOSORBENT ASSAY |