Author/Editor     Avčin, T; Ambrožič, A; Kuhar, M; Kveder, T; Rozman, B
Title     Anticardiolipin and anti-beta2 glycoprotein 1 antibodies in sera of 61 apparently healthy children at regular preventive visits
Type     članek
Source     Rheumatol Oxf Print
Vol. and No.     Letnik 40, št. 5
Publication year     2001
Volume     str. 565-73
Language     eng
Abstract     Objectives: To determine anticardiolipin (aCL) and anti-beta(2) glycoprotein I antibodies (anti-beta(2)GPI) in apparently healthy children and express the cut-off levels in concentrations of monoclonal antibodies, and to compare the mean values and frequencies of aCL and anti-beta(2)GPI in children with those in blood donors. Methods: Blood samples were collected from 29 preschool children and 32 adolescents during their routine preventive follow-up visits. The control group consisted of 52 blood donors. aCL and anti-beta(2)GPI were assayed by an ELISA method. Two monoclonal beta(2)GPI-dependent aCL (HCAL and EY2C9) were used as calibrators. Results: The estimated cut-off values for immunoglobulin G (IgG) and immunoglobulin M (IgM) aCL, expressed in concentrations of monoclonal antibodies and standardized international units (GPL/MPL units), were 13.9 ng/ml (7.6 GPL) and 33.1 ng/ml (3.3 MPL) for preschool children, 13.5 ng/ml (7.2 GPL) and 36.9 ng/ml (4.0 MPL) for adolescents, and 14.4 ng/ml (8.0 GPL) and 42.6 ng/ml (5.1 MPL) for blood donors. No statistically significant differences in the mean values for IgG and IgM aCL were found between the age groups. The mean value of IgA aCL was significantly higher in blood donors than in preschool children and adolescents (P<0.037 and P<0.025 respectively). Seven (11.4%) of 61 apparently healthy children had low positive values for aCL (IgG for all seven). The estimated cut-off values for IgG and IgM anti-beta(2)GPI were 4.2 and 13.1 ng/ml respectively for preschool children, 3.2 and 13.1 ng/ml for adolescents, and 2.9 and 20.5 ng/ml for blood donors. The mean value for IgG anti-beta(2)GPI was found to be higher in preschool children than in adolescents and blood donors (P<0.0001 and P<0.0001). (Abstract truncated at 2000 characters)
Descriptors     ANTIBODIES, ANTICARDIOLIPIN
GLYCOPROTEINS
ANTIBODIES, MONOCLONAL
CHILD, PRESCHOOL
ADOLESCENCE
ENZYME-LINKED IMMUNOSORBENT ASSAY
IGG
IGM