Author/Editor		Masson, Patrick; Nachon, Florian; Xie, Weihua; Froment, Marie-Therese; Weingand-Ziade, Alexandra; Goeldner, Maurice; Lockridge, Oksana
Title		The peripheral anionic site of butyrylcholinesterase: from facts to functions
Translated title		Periferno anionsko mesto na butilholinesterazi: od dejstev k vlogi
Type		članek
Source		Acta Biol Slov
Vol. and No.		Letnik 43, št. 1-2
Publication year		2000
Volume		str. 137-42
Language		eng
Abstract		Kinetic, inhibition, labelling and stability studies were carried out to probe the peripheral anionic site (PAS) of human butyrylcholinesterase. It was found that a) D70 and Y332 are key components of the PAS, b) there is cross-talk between tha PAS and the catalytic site, c) the PAS controls the hydration of the active site gorge.
Summary		Vlogo anionskega perifernega mesta človeške butilholinesteraze smo raziskovali s pomočjo kinetičnih študij, inhibicije, označevanja in stabilnosti. Ugotovili smo da: a) sta najbolj pomembna aminokislinska ostanka asparginska kislina na mestu 70 in tirozin na mestu 332, b) obstaja navzkrižna povezanost med perifernim anionskim mestom in katalitičnim mestom encima, c) periferno anionsko mesto nadzoruje hidracijo mesta, kjer leži aktivni center encima.
Descriptors		BUTYRYLCHOLINESTERASE BINDING SITES CHO CELLS CATALYSIS POLYMERASE CHAIN REACTION RECEPTOR AGGREGATION