Author/Editor		Praprotnik, Sonja
Title		Specifičnost in patogenetski pomen protiteles proti endotelnim celicam malega in velikega žilja
Translated title		Specificity and pathogenic significance of anti-endothelial cell antibodies against microvascular and macrovascular endothelial cells
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		2001
Volume		str. 103
Language		slo
Abstract		Antibodies against endothelial cells (anti-endothelial cell antibody -AECA) have been detected in sera of patients with various diseases, which share a varying degree of vessel wall damage. Although there are some unequivocal reports of a pathogenic role of AECA in systemic vasculitis, the mechanisms underlying the effect of AECA on endothelium are still poorly understood. The heterogeneous group of disease in which AECA have been described differ also in the selective involvement of peculiar anatomical vascular districts. Endothelial cells from micro- and macrovasculature differ in their phenotypical and functional characteristic. Therefore, the attractive hypothesis that AECA from different sources recognize different types of endothelial cell target molecules that are correlated to the origin of the disease was advocated. Affinity purified AECA F(ab)2 portion from patients with throrhbotic thrombocytopenic purpura bound and activated microvascular endothelial cells (human bone marrow endothelial cells) and not large vessel endothelial cells. It is worth noting that thrombotic thrombocytopenic purpura is characterized by small vessels thrombosis only. Human monoclonal AECA IgG from Takayasu's artertis patient possessed high activity against macrovascular endothelial cells (human umbilical vein endothelial cells), but none against microvascular endothelial cells, which is again in concordance with the pathological features of the disease. (Abstract truncated at 2000 characters)
Descriptors		ENDOTHELIUM, VASCULAR AUTOANTIBODIES PURPURA, THROMBOTIC THROMBOCYTOPENIC THROMBOCYTOPENIA TAKAYASU'S ARTERITIS HEPARIN CELLS, CULTURED IMMUNOGLOBULINS, FAB INTERLEUKIN-6 VON WILLEBRAND FACTOR ANTIBODIES, MONOCLONAL IMMUNOHISTOCHEMISTRY