Author/Editor		Ivanuša, Teodora; Beravs, Katarina; Čemažar, Maja; Jevtič, Vladimir; Demšar, Franci; Serša, Gregor
Title		MRI macromolecular contrast agents as indicators of changed tumor blood flow
Translated title		Uporaba kontrastno povdarjenega dinamičnega MR slikanja za spremljanje pretoka krvi v tumorjih
Type		članek
Source		Radiol Oncol
Vol. and No.		Letnik 35, št. 2
Publication year		2001
Volume		str. 139-47
Language		eng
Abstract		Background. A rapid mapping technique derived from dynamic contrast enhanced MRI data was used to identify and characterize reduction of blood flow in fibrosarcoma SA-1 tumors treated either by application of electric pulses or vinblastine. Materials and methods. Tissue permeability surface area product (PS) and fractional blood volume (BV) were calculated on a pixel-by-pixel basis using dynamic MRI intensity data after administration of gadomer - 17 or polylysine-Gd-DTPA; prototypic macromolecular contrast agents designed for blood pool enhancement. PS and BV values of untreated tumors were compared to those of tumors treated by local application of 8 electric pulses (amplitude/distance ratio, 1300 V/cm; duration, 100 us, frequency, 1 Hz) percutaneously to the tumor or by systemic administration of vinblastine (2.5 mg/kg). Results. Both treatments transiently, but significantly reduced tumor blood flow, application of electric pulses to the tumors being by 40% more effective in reducing tumor blood flow than systemic administration of vinblastine. PS and BV values derived with polylysine-Gd-DTPA-enhanced MRI were lower compared to those with gadomer-17, due to larger molecular size. Interestingly, Gd-DTPA-enhanced MRI did not show any significant changes of PS and BV between untreated and treated tumors. Conclusion. This study demonstrates that dynamic contrast enhanced MRI can be effectively used to qualitatively monitor tumor blood flow, and quantitatively by means of BV and PS.
Summary		Izhodišče. Dinamično slikanje z magnetno resonanco je diagnostična metoda, ki omogoča prikaz in vrednotenje prekrvavljenosti tumorjev. Namen naloge je bil z makromolekularnimi kontrastnimi sredstvi spremljati zmanjšanje pretoka krvi v mišjih fibrosarkomskih tumorjih po aplikaciji električnih pulzov ali zdravljenju z vinblastinom. Materiali in metode. Permeabilnost tumorskega žilja (PS) in prekrvavljenost tumorskega tkiva (BV) smo ocenjevali na nivoju točkovnih elementov MR signala po aplikaciji dveh prototipov makromolekularnih kontrastnih sredstev: gadomer-17 in polilizin-Gd-DTPA, ki za razliko od nizko molekularnega gadolinija (Gd-DTPA) omogočajo prikaz tumorskega žilja. PS in BV netretiranih tumorjev smo primerjali z PS in BV vrednostimi tumorjev, katerim smo aplicirali električne pulze ali jih zdravili z vinblastinom. Rezultati. Oba načina zdravljenja sta značilno zmanjšala pretok krvi v tumorjih. Aplikacija električnih pulzov pa je bila za 40% učinkovitejša v zmanjšanju pretoka krvi v tumorjih kot sistemska aplikacija vinblastina. PS in BV vrednosti za polilizin-Gd-DTPA so bile nižje kot vrednosti za gadomer-17 (30 kDa), kar pojasnjujemo z velikostjo molekul polilizina-Gd-DTPA (50 KDa). Zanimivo je, da PS in BV vrednosti kontrastno podarjenega dinamičnega MR slikanja z gadolinijem niso pokazale značilne razlike med netretiranim in zdravljenimi tumorji. Zaključek. Rezultati raziskave utemeljujejo uporabo kontrastno povdarjenega dinamičnega MR slikanja za spremljanje pretoka krvi v tumorjih in kvantitativno določanje PS in BV vrednosti.
Descriptors		SARCOMA, EXPERIMENTAL VINBLASTINE ELECTROPORATION MAGNETIC RESONANCE IMAGING MICE DRUG DELIVERY SYSTEMS CONTRAST MEDIA GADOLINIUM