| Author/Editor | Cvenkel, Barbara | |
| Title | Vpliv antiglavkomske terapije na izražanje antigenov HLA-DR na epitelijskih celicah veznice | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Medicinska fakulteta | |
| Publication year | 2001 | |
| Volume | str. 74 | |
| Language | slo | |
| Abstract | Purpose: To analyse the expression of inflammatory markers HLA-DR on conjunctival epithelial cells in patients taking topical antiglaucoma therapy. Methods: The impression cytology specimens were obtained from 10 patients taking no topical medication and from 39 patients with uncontrolled glaucoma referred for filtration surgery. In none of the patients was an ocular surface inflammation present as evaluated by slit lamp examination, tear break up time, and Schirmer's test. The specimens were collected preoperatively, 3 months, and 6 months after surgery. The expression of HLA-DR on T lymphocytes and epitheliai cells was analysed by flow cytometry. Results: A significant increase in the level of HLA-DR fluorescence on epithelial cells was found preoperatively in patients with glaucoma compared to control group (P = 0,002). Antiglaucoma therapy for three months or more with at least two topical medications induced significant subclinical inflammation which persisted regardless of the duration of therapy. The increased percentage of HLA-DR positive epithelial cells and T lymphocytes in the control group (P = 0,006 and P = 0,01) mainly reflected the greater number of collected cells, and not the level of inflammation. A significant increased expression of HLA-DR on T lymphocytes was detected 3 months after surgery (P = 0,04). Higher percentage and fluorescence intensity of reactive T lymphocytes was associated with higher percentage and fluorescence intensity of reactive epithelial cells three months after surgery. Six months after surgery the level of HLA-DR fluorescence on epithelial cells and T lymphocytes decreased but remained elevated versus preoperative levels. (Abstract truncated at 2000 characters) | |
| Descriptors | GLAUCOMA CONJUNCTIVA HLA-DR ANTIGENS T-LYMPHOCYTES FLOW CYTOMETRY TREATMENT OUTCOME |