Author/Editor		Gobec, S; Urleb, S; Simčič, S; Wraber, B
Title		Synthesis and modulation of cytokine production by two new adamantane substituted acyclic desmuramyldipeptide analogs
Type		članek
Source		Pharmazie
Vol. and No.		Letnik 56, št. 7
Publication year		2001
Volume		str. 523-6
Language		eng
Abstract		Two new adamantyl-desmuramyldipeptides LK 415 and LK 517 with 1-adamantylcarboxamido moiety as a replacement for muramyldipeptide's N-acetylglucosamine grafment were synthetized. Their efficacy to modulate the production of cytokines was measured in vitro in ionomycin and phorbol-12-myristate-13-acetate (PMA) activated cultures of human peripheral blood mononuclear cells (PBMC), co-incubated with the substances tested. The results were compared with the activity of muramyldipeptide (MDP). All three substances are strong up-regulators of IL-12 synthesis and hence of the IFNgamma synthesis as well. While MDP and LK 415 are relatively inffective in modulation of IL-2, IL-4 and IL-10 production in vitro, the synthesis of all three cytokines is considerably up-regulated when peripheral blood mononuclear cells are co-incubated with LK 517.It seems likely that the introduction of the diethyl phosphonate moiety into LK 617 is of great importance for the augmented T-cell cytokine production.
Descriptors		ACETYLMURAMYL-ALANYL-ISOGLUTAMINE CYTOKINES MONOCYTES IONOMYCIN TETRADECANOYLPHORBOL ACETATE INTERLEUKIN-12 INTERLEUKIN-2 INTERLEUKIN-4 INTERLEUKIN-10 TUMOR NECROSIS FACTOR INTERFERON TYPE II