| Author/Editor | Kopač, Igor | |
| Title | Učinkovitost in delovanje retrakcijskih sredstev za začasni odmik dlesne | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Medicinska fakulteta | |
| Publication year | 2001 | |
| Volume | str. 78 | |
| Language | slo | |
| Abstract | Temporary displacement of the free gingival margin is vital for identification of the finish line of the preparation in a die plaster model. This enables accurate modelling of the margins of fixed restorations, which in turn ensures a proper relationship between crowns and gingival tissue. There are several methods for enlarging of sulcular space before impression making in clinical practice: mechanical and chemo-mechanical free gingival displacement, electrosurgery and rotary gingival curettage. However, all the methods have more or less adverse effects on the gingiva. This study only examined the effects of the chemical agents that are most commonly used for the impregnation of cotton cords, i.e. aluminum chloride of two concentrations, aluminum sulfate and tetrahydrozoline. The purpose of the study was to determine which agent caused sufficient displacement of free gingiva suitable for impression making while exerting the least inflammatory response in the gingival tissue and the least notable changes at the cell level. The study examined the effects of chemical agents on fibroblasts and keratinocytes in cell cultures and on dogs in vivo. Standard methods of cell viability measurements, such as cell viability test, cell survival test and colorimetric MTT test, were used on V-79 fibroblasts. The study was supplemented by scanning and transmision electron microscopy on rat keratinocytes. This method enabled us to qualitatively evaluate the differences between the control group and those treated with two agents, i.e. the most, and the least aggressive ones. (Abstract truncated at 2000 characters). | |
| Descriptors | GINGIVA FIBROBLASTS KERATINOCYTES ALUMINUM COMPOUNDS TETRAZOLES RATS DOGS DENTURE, PARTIAL, FIXED CELL SURVIVAL CELL LINE MICROSCOPY, ELECTRON IMAGE PROCESSING, COMPUTER-ASSISTED COLORIMETRY GINGIVAL RECESSION BIOMETRY |