| Author/Editor | Milivojevič, Nataša | |
| Title | Učinki N-tert-butil-alfa-fenil-nitrona na modelu kognitivne disfunkcije pri podgani | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Medicinska fakulteta | |
| Publication year | 2001 | |
| Volume | str. 72 | |
| Language | slo | |
| Abstract | It has been shown, that chronic treatment with free radical scavenger N-tert-butyl-alpha-phenylnitrone (PBN) improves cognitive performance of aged rats. This finding was attributed to neuroprotective effects of PBN due to its free radical scavenging activity. Recently it has been found that PBN also exhibits anticholinesterase activity. In the model of diisopropylphosphorofluoridate-induced seizures, PBN partially protected AChE from critical irreversible phosphorylation (Milatović et al., 2000). Anticholinesterase activity of PBN was disclosed by in vitro studies where PBN inhibited rat brain AChE as mixed competitive/noncompetitive inhibitor with Ki and Ki' of 0.693 and 3.874 mM, respectively. It is known that reversible inhibitors of acetylcholinesterase improve spatial learning and memory in animal models of cognitive impairment. This raised the question whether the beneficial effects of PBN on cognitive performance could be explained by its anticholinesterase activity. In the present study I therefore compared the effects of PBN to the effects of reversible anticholinesterases physostigmine and tacrine on the impairment of spatial learning and memory induced by muscarinic antagonist scopolamine in the Morris water maze paradigm and on scopolamine induced locomotor hyperactivity in the open field behavioral experiment. In addition, using gene expression analysis by in situ hybridization method I examined whether PBN can antagonize the augmenting effects of scopolamine on the induction of immediate early gene (IEG; c jun) expression by SKF-82958. (Abstract truncated at 2000 characters) | |
| Descriptors | COGNITION DISORDERS FREE RADICAL SCAVENGERS ACETYLCHOLINESTERASE SCOPOLAMINE PHYSOSTIGMINE RATS, WISTAR IN SITU HYBRIDIZATION GENES, JUN BEHAVIOR, ANIMAL MEMORY GENE EXPRESSION TACRINE RNA, MESSENGER CORPUS STRIATUM |