Author/Editor		Kavalar, R; Sarčević, B; Spagnoli, GC; Šeparović, V; Samija, M; Terracciano, L; Heberer, M; Juretič, A
Title		Expression of MAGE tumour-associated antigens is inversely correlated with tumour differentiation in invasive ductal breast cancers: an immunohistochemical study
Type		članek
Source		Virchows Arch
Vol. and No.		, št. 439
Publication year		2001
Volume		str. 127-31
Language		eng
Abstract		MAGE (Melanoma antigen E) family gene products encompass tumour-associated antigens (TAAs) recognised by human leukocyte antigen (HLA-restricted specific T-cells. Agents inducing DNA demethylation, an event typically detectable in cellular de-differentiation processes, were shown to induce the exoression of MAGE genes. By using a monoclonal antibody specific for MAGE family gene products, we have studied the expression of these TAAs in a group of 144 patients with invasive ductal breast cancers. Immunohistochemical data were correlated with tumour differentiation, lymphatic vessel invasion, oestrogen receptor expression, intratumoural necrosis, lymphocytic infiltration, perineural invasion, tumour microcalcifications and axillary lymp node metastases. MAGE immunoreactivity was undetectable in non/neoplastic cells. In poorly differentiated cancers positive staining was observed in 30/63 cases (47,6%) as compared with 13/51 (25.4%) and 5/30 (16.6%) in moderately and well-differentiated tumours, respectively (P<0.05). In addition, MAGE immunoreactivity was significantly correlated with lymphatic vessel invasion and intratumoural necrosis. Moreover, a significant inverse relationship with oestrogen receptor expression was also observed. (Abstract truncated at 2000 characters)
Descriptors		BREAST NEOPLASMS CARCINOMA, INFILTRATING DUCT ANTIGENS, TUMOR-ASSOCIATED, CARBOHYDRATE IMMUNOHISTOCHEMISTRY IMMUNOPHENOTYPING HLA ANTIGENS ANTIGENS, CD3 ANTIGENS, CD4 ANTIGENS, CD8 ANTIGENS, CD20