Author/Editor		Zorc, Marjeta; Vraspir-Porenta, Olga; Zorc-Pleskovič, Ruda; Radovanović, Ninoslav; Cijan, Andrej; Milosavljević, Aleksandar; Petrovič, Danijel
Title		Myocytes' apoptosis and proliferation in endomyocardial biopsy as prognostic factors in terminal heart failure
Type		članek
Source		Pflugers Arch
Vol. and No.		Letnik 442, št. 6 Suppl 1
Publication year		2001
Volume		str. R163-4
Language		eng
Abstract		The aim of the preliminary study was to evaluate the role of apoptosis and proliferation of myocytes in order to predict the prognosis and optimal treatment of patients with end stage dilated cardiomyopathy. Endomyocardial biopsy was performed during open-heart surgery (reductive annuloplasty of double orifice) in 19 patients with end-stage dilated cardiomyopathy. The terminal deoxynucleotidyl transferase d-UTP-biotin nick-end labelling (TUNEL) method was used for the detection of apoptosis and immunohistochemical methods were used for the evaluation of inhibitor of apaptosis such as proto-oncogene Bcl-2 (Bcell: lymphoma gene), and proliferative markers such as proliferation cell nuclear antigen (PCNA) and Ki-67 proliferative antigen. The increased percentage of apoptotic myocytes, and decreased expression of bcl-2 is associated with earlier death after surgery. Increased expression of proliferation markers of myocytes in patients who survived seven years after surgery compared to those who died within three years suggest that adult cardiomyocytes are not terminally differentiated and this might represent potential growth reserve of the diseased heart. Based an our preliminary study -may conclude that myocytes apoptosis and proliferative activity might help us to predict the prognosis and optimal treatment of patients with end-stage dilated cardiomyopathy.
Descriptors		HEART FAILURE, CONGESTIVE MYOCARDIUM APOPTOSIS PROGNOSIS IMMUNOHISTOCHEMISTRY