Author/Editor		Dermastia, Marina; Chourey, Prem S
Title		Expression of cell wall invertase from maize cell suspension culture after a long term chelation of extracellular calcium
Type		članek
Source		Phyton-ann Rei Bot A
Vol. and No.		Letnik 41, št. 1
Publication year		2001
Volume		str. 63-74
Language		eng
Abstract		The cell wall-bound form of invertase (EC 3.2.1.26) from a maize cell suspension culture was affected by removal of extracellular calcium, over time, at RNA, protein and enzyme activity level. The increase in enzyme activity was detected in cells treated with 5 mM EGTA, which preferentially chelates extracellular Ca2+. Although the enzyme activity of EGTA-treated cells was low and similar to the control after 24 hours of incubation, it started to increase soon thereafter. It reached the maximum after 96 hours, when a decline of invertase activity had already been detected in the control samples. The amount of the invertase protein corresponds to its determined specific activity. Calcium depletion also induced Incw1 mRNA accumulation, and the concentration of Incw1 mRNA in EGTA-treated cells was 1.7 times higher than in the control after the first 24 hours. It remained high in the next 24 hours and slowly decreased throughout a 96 hour-incubation. At the end of the experiment the transcript
Descriptors		CORN CELL WALL GLYCOSIDE HYDROLASES EGTAZIC ACID CALCIUM CELLS, CULTURED RNA, PLANT PLANT PROTEINS ELECTROPHORESIS, POLYACRYLAMIDE GEL