Author/Editor		Kos, J; Sekirnik, A; Kopitar, G; Cimerman, N; Kayser, K; Stremmer, A; Fiehn, W; Werle, B
Title		Cathepsin S in tumours, regional lymph nodes and sera of patients with lung cancer: relation to prognosis
Type		članek
Source		Br J Cancer
Vol. and No.		Letnik 85, št. 8
Publication year		2001
Volume		str. 1193-200
Language		eng
Abstract		Cysteine proteinase cathepsin S (Cat S) is expressed mainly in lymphatic tissues and has been characterised as a key enryme in major histocompatibility complex class II (MHC-II) mediated antigen presentation. Cat S has been measured in tissue cytosols of lung parenchyma, lung tumours and lymph nodes and in sera of patients with lung tumours and of healthy controls, by specfic enzyme-linked immunosorbeM assay (ELISA). A difference in Cat S level was found between tumour and adjacent control tissue cytosols of 60 lung cancer patients (median 4.3 vs. 2.8 ng mg-protein). In lymph nodes obtained from 24 patients of the same group, the level of Cat S was significantly higher than in tumours or lung parenchyma (P < 0.001). Additionally, significantly higher levels were found in non-infiHrated than in infiltrated lymph nodes (median 16.6 vs 7.5 ng mg- protein). Patients with low levels of Cat S in tumours and lung parenchyma exhibited a significantly higher risk of death than those with high levels of Cat S (P < 0.025 -tumours; P < 0.002 - parenchyma). Immunohistochemical analysis (IHA) of lung parenchyma revealed a staining reaction in alveolar type II cells, macrophages and bronchial epithelial cells. In regional lymph node tissue, strong staining of Cat S was found in lymphocytes and histiocytes. Nevertheless, Cat S was detected also in tumour cells, independently of their origin. Our results provide evidence that Cat S may be involved in malignant progression. Its role, however, differs from that of the related Cats B and L and could be associated with the immune response rather than with remodelling of extracellular matrix.
Descriptors		CATHEPSINS LUNG NEOPLASMS LYMPH NODES PROGNOSIS ANTIBODY SPECIFICITY CYTOSOL ENZYME-LINKED IMMUNOSORBENT ASSAY IMMUNOHISTOCHEMISTRY