| Author/Editor | Milatovič, Dejan; Živin, Marko; Gupta, Ramesh C; Dettbarn, Wolf D | |
| Title | Alterations in cytochrome c oxidase activity and energy metabolites in response to kainic acid-induced status epilepticus | |
| Type | članek | |
| Source | Brain Res | |
| Vol. and No. | Letnik 912, št. 1 | |
| Publication year | 2001 | |
| Volume | str. 67-78 | |
| Language | eng | |
| Abstract | The effects of kainic acid (KA)-induced limbic seizures have been investigated on cytochrome c oxidase (COx) activity, COx subunit IV mRNA abundance, ATP and phosphocreatine (PCr) levels in amygdala, hippocampus and frontal cortex of rat brain. Rats were killed either 1 h, three days or seven days after the onset of status epilepticus (SE) by CO(2) and decapitation for the assay of COx activity and by head-focused microwave for the determination of ATP and PCr. Within 1 h COx activity and COx subunit IV mRNA increased in all brain areas tested between 120% and 130% of control activity, followed by a significant reduction from control, in amygdala and hippocampus on day three and seven, respectively. In amygdala, ATP and PCr levels were reduced to 44% and 49% of control 1 h after seizures. No significant recovery was seen on day three or seven. Pretreatment of rats with the spin trapping agent N-tert-butyl-alpha-phenylnitrone (PBN, 200 mg kg(-1), i.p.) 30 min before KA administration had no effect on SE, but protected COx activity and attenuated changes in energy metabolites. Pretreatment for three days with the endogenous antioxidant vitamin E (Vit-E, 100 mg/kg, i.p.) had an even greater protective effect than PBN. Both pretreatment regimens attenuated KA-induced neurodegenerative changes, as assessed by histology and prevention of the decrease of COx subunit IV mRNA and COx activity in hippocampus and amygdala, otherwise seen following KA-treatment alone. These findings suggest a close relationship between SE-induced neuronal injury and deficits in energy metabolism due to mitochondrial dysfunction. | |
| Descriptors | STATUS EPILEPTICUS KAINIC ACID CYTOCHROME-C OXIDASE RNA, MESSENGER ADENOSINE TRIPHOSPHATE RATS, SPRAGUE-DAWLEY HISTOCYTOCHEMISTRY PHOSPHOCREATINE AMYGDALOID BODY HIPPOCAMPUS CEREBRAL CORTEX VITAMIN E |