Author/Editor		Gašperšič, Rok; Koritnik, Blaž; Eržen, Ida; Sketelj, Janez
Title		Muscle activity-resistant acetylcholine receptor accumulation is induced in places of former motor endplates in ectopically innervated regenerating rat muscles
Type		članek
Source		Int J Dev Neurosci
Vol. and No.		Letnik 19, št. 3
Publication year		2001
Volume		str. 339-46
Language		eng
Abstract		Expression of acetylcholine receptors (AChRs) in the extrajunctional muscle regions, but not in the neuromuscular junctions, is repressed by propagated electric activity in muscle fibers. During regeneration, subsynaptic-like specializations accumulating AChRs are induced in new myotubes by agrin attached to the synaptic basal lamina at the places of former motor endplates even in the absence of innervation. We examined whether AChRs still accumulated at these places when the regenerating muscles were ectopically innervated and the former synaptic places became extrajunctional. Rat soleus muscles were injured by bupivacaine and ischemia to produce complete myofiber degeneration. The soleus muscle nerve was permanently severed and the muscle was ectopically innervated by the peroneal nerve a few millimeters away from the former junctional region. After 4 weeks of regeneration, the muscles contracted upon nerve stimulation, showed little atrophy and the cross-section areas of their fibers were completely above the range in non-innervated regenerating muscles, indicating successful innervation. Subsynaptic-like specializations in the former junctional region still accumulated AChRs (and acetylcholinesterase) although no motor nerve endings were observed in their vicinity and the cross-section area of their fibers clearly demonstrated that they were ectopically innervated. We conclude that the expression of AChRs at the places of the former neuromuscular junctions in the ectopically innervated regenerated soleus muscles is activity-independent.
Descriptors		MOTOR ENDPLATE MUSCLE, SKELETAL RECEPTORS, NICOTINIC REGENERATION RATS, WISTAR ACETYLCHOLINESTERASE MOTOR NEURONS MUSCLE FIBERS NERVE ENDINGS MUSCLE DENERVATION