Author/Editor		Pšeničnik, M; Veranič, P
Title		Autophagy-related vacuoles in mouse gallbladder epithelium
Type		članek
Source		Folia Histochem Cytobiol
Vol. and No.		Letnik 39, št. 1
Publication year		2001
Volume		str. 3-8
Language		eng
Abstract		The mouse gallbladder epithelial cells contain very heterogeneous vacuolar population. In an attempt to classify these vacuoles we identified NADPase and TPPase activity as well as the location of HRP which is used as the endocytotic marker. The results of the present study show that the vacuoles can be classified into three categories: (1) the vacuoles predominantly containing loose membrane coils related to the nascent autophagic vacuoles, (2) vacuoles containing densely packed membranes and exhibiting a positive HRP reaction, indicating the convergence of endocytotic and autophagic pathhway, and (3) vacuoles composed of degraded membrane structures and containing the reaction product of NADPase activity, showing that the fusion of the lysosomes with the autophagosome-endosome took place. The highly developed cis, medial and trans Golgi compartments reflect the biosynthetic and endocytotic activity of the gallbladder epithelium.
Descriptors		GALLBLADDER EPITHELIUM AUTOPHAGOCYTOSIS VACUOLES MICE HISTOCYTOCHEMISTRY MICROSCOPY, ELECTRON NADH DEHYDROGENASE THIAMINE PYROPHOSPHATASE GOLGI APPARATUS ENDOCYTOSIS HORSERADISH PEROXIDASE