Author/Editor     Jevnikar, Nataša
Title     Povezava med gostoto makrofagov v periapikalnem granulomu in klinično sliko
Type     monografija
Place     Ljubljana
Publisher     Medicinska fakulteta
Publication year     2001
Volume     str. 62
Language     slo
Abstract     Distinction of lesions is based on clinical symptoms and intraoral x-ray. They can roughly be divided on acute apical parodontitis and chronic apical parodontitis, where three different patohistologic types are described: periapical granuloma (PG), radicular cyst and apical scar. The most common type is PG, that has been found in 59%. Patohistologic structure of PG consists of: granulation tissue with many capillaries, fibroblasts and connective fibers, as well as inflammatory infiltrate. Lymphocytes T and B most commonly predominate in inflammatory infiltrate, however, macrophages can rarely be found as main type. Macrophages fagocyte bacteria. In addition, they damage and disintegrate periapical tissue by releasing bioactive substances (cytokines, free radicals, proteolytic encymes). A topographically oriented study was conducted to find out whether there is a differences in patohistological structure of PG regarding the clinical symptoms. It was our hypothesis that the highest density of macrophages in acute exacerbation of PG is in the outer third and therefore expansion of PG in humans is mainly due to the action of macrophages.Thirty three human periapical granulomas were obtained either by extraction or during endodontic surgical procedure. PG were assigned to the following groups: symptomatic-non symptomatic, endodontically treated-non treated. Following the fixation, PG were peeled off the root surface and the part adjacent to apex marked with ink. This enabled precise topographical orientation. Specimes comprised 5 step serial paraffin embedded sections. One section was HE stained, while the others being subjected to immunoperoxidase staining. Monoclonal antibodies CD 68 were used to specifically label vessel endothelia. (Abstract truncated at 2000 characters).
Descriptors     PERIAPICAL ABSCESS
MACROPHAGES
ANTIGENS, CD3
ANTIGENS, CD31
T-LYMPHOCYTES
B-LYMPHOCYTES
IMMUNOHISTOCHEMISTRY
ACUTE DISEASE
AGE FACTORS
ANTIBODIES, MONOCLONAL
LYMPHOCYTE COUNT