Author/Editor		Barborič, Matjaž; Nissen, Robert M; Kanazawa, Satoshi; Jabrane-Ferrat, Nabila; Peterlin, Matija B
Title		NF-kappaB binds P-TEFb to stimulate transcriptional elongation by RNA polymerase II
Type		članek
Source		Mol Cell
Vol. and No.		Letnik 8, št. 2
Publication year		2001
Volume		str. 327-37
Language		eng
Abstract		To stimulate transcriptional elongation of HIV-1 genes, the transactivator Tat recruits the positive transcription elongation factor b (P-TEFb) to the initiating RNA polymerase II (RNAPII). We found that the activation of transcription by ReIA also depends on P-TEFb. Similar to Tat, ReIA activated transcription when tethered to RNA. Moreover, TNF-alpha triggered the recruitment of P-TEFb to the NF-KB-regulated IL-8 gene. While the formation of the transcription preinitiation complex (PIC) remained unaffected, DRB, an inhibitor of P-TEFb, prevented RNAPII from elongating on the IL-8 gene. Remarkably, DRB inhibition sensitized cells to TNF-alphainduced apoptosis. Thus, NF-KB requires P-TEFb to stimulate the elongation of transcription and P-TEFb plays an unexpected role in regulating apoptosis.
Descriptors		NF-KAPPA B PROTEIN-SERINE-THREONINE KINASES RNA POLYMERASE II TRANSCRIPTION, GENETIC APOPTOSIS DNA-BINDING PROTEINS DICHLORORIBOFURANOSYLBENZIMIDAZOLE GENE PRODUCTS, TAT GENES, RECESSIVE HIV-1 INTERLEUKIN-8 NUCLEIC ACID SYNTHESIS INHIBITORS RECOMBINANT FUSION PROTEINS TRANS-ACTIVATION (GENETICS) TUMOR CELLS, CULTURED TUMOR NECROSIS FACTOR