Author/Editor		Bunc, Gorazd; Kovačič, Srečko; Strnad, Simona
Title		Evaluation of functional response of cerebral arteries by a new morphometric technique
Type		članek
Source		Autonom Neurosci Basic Clin
Vol. and No.		Letnik 93
Publication year		2001
Volume		str. 41-7
Language		eng
Abstract		The contractile response of the rabbit basilar artery under four conditions was determined: (1) response in a resting condition without exclusion of the sympathetic nervous system (control group I); (2) response in a resting condition with a-adrenoceptor blockade (group II); (3) response to subarachnoid haemorrhage (SAH) (group III); and (4) response to SAH with a-adrenoceptor blockade (group IV). It was also ascertained whether it was possible to measure contractile response using a new morphometric method. Vessels were prepared by intracardial perfusion fixation, stained by haematoxylin and eosin, and the length of the intimal corrugations were measured by computer image analysis. Two procedures were followed in order to express the intensity of intimal corrugation, indicating the contractibility of the basilar arteries: (1) the corrugation coefficient (CC) of the basilar artery intima was estimated by dividing the precisely measured length of the intimal corrugations by the length of the measured vessel wall section of the vessel cross-sections (obtained histologically); (2) the lumen reduction coefficient (LRC) of the basilar artery was determined by dividing the "ideal" lurninal area (calculated from the total length of the intimal circumference) by the real luminal cross-section area. (Abstract truncated at 2000 characters)
Descriptors		SUBARACHNOID HEMORRHAGE ADRENERGIC ALPHA-ANTAGONISTS BASILAR ARTERY VASOCONSTRICTION IMAGE PROCESSING, COMPUTER-ASSISTED TUNICA INTIMA BIOMETRY RABBITS