Author/Editor		Berovič, Marin; Knežević, Miomir
Title		The use of tetracycline in submerge Pleurotus saca cultivation, a possibility for large scale production
Type		članek
Source		In: Jishan H, editor. Proceedings of the international symposium on Asian science and technology and development; 1995; Cansha. Chansha: Central south university of technology press,
Publication year		1995
Volume		str. 201-6
Language		eng
Abstract		The main idea of our research work on the submerged cultivation of Pleurotus saca was to establish the reclinological possibility of a large production of fungal vegetative inoculum, to be used for commercial production of the oyster mushroom on solid substrate. To prevent often bacterial infection used for commercial production, the effect of various tetracycline concentrations was tested. During the experimental work we found that the addition of tetracycline from 3 to 6 X 10-3g l-1, did not stop bacterial infection, additions from 6. 5 to 8:0 X 10-3g l-1 prevented-it efficiently, promoting oxygen consumption and the growth of biomass, while amounts from 8 to 12 X 10-3g l-1 and higher inhibited it. If the infection occurred during the cultivation process,even 12 to 16 X 10-3g l-1 of tetracyclyne added to cultivation broth later couldn't prevent infection. In all experiments fungus Pleurotus saca strain MZKIBK G23 and substrate beer wort (17% dry weight) diluted with demineralised water 1 : 1 were used. All experiments proceeded in 10 l laboratory bioreactor. During cultivation (T= 25 degrees C, air flow 5 l/min, mixing 500 rpm) the fungal morphology changed from small hyphal agglomerates to very thick filamentous growth during the late tog phase. The cultivation was stopped when the broth was too viscous, so that the oxygen transport was reduced and even more intensive stirring (800 rpm), not increased its concentration. The use of axial mixers and draft tubes is recommended. Tetracycline concentrations were detected using HPLC technique. After 100 hours no detectable amounts of tetracycline were measured in cultivation broth while biomass of submerge cultivated Pleurotus saca increased 200% in average.
Descriptors		AGARICALES TETRACYCLINE BACTERIA OXYGEN CONSUMPTION BIOMASS CULTURE MEDIA