Author/Editor		Maček, Marjeta
Title		Granulocitni antigeni in njihov pomen v transfuzijski medicini
Type		monografija
Place		Ljubljana
Publisher		Zavod Republike Slovenije za transfuzijo krvi
Publication year		2001
Volume		str. 70
Language		slo
Abstract		The neutrophil-specific antigens HNA-1a, HNA-1b, and HNA-1c are clinically most important antigens on the neutrophil membrane. They are identified as polymorphic forms of FcyRIIIb receptor, which is determined biochemicaly and on the molecular level. Antibodies against granulocyte antigens can be produced following the transfusion of granulocytes, in pregnancy and following bone marrow transplantation. They cause alloimmune neonatal neutropenia, immune neutropenia after bone marrow transplantation, refractoriness to granulocyte transfusion and transfusion reactions as TRALI (Transfusionrelated acute lung injury) syndrom and febrile nonhemolytic transfusion reaction. If own granulocyte antigens are the target of the immune response, the autoimmune neutropenia and drug-induced neutropenia occur. For the diagnosis of these clinical conditions determination of granulocyte antibodies is essential. Granulocyte antibodies are mostly determined by the immunofluorescence test, agglutination test and MAIGA (Monoclonal antibody-specific imobilisation of granulocyte antigens) assay. The latter allows determination of the granulocyte antibody specificity. Antigens HNA-1a, HNA-1b and HNA-1c can be genotyped by polymerase chain reaction with sequence specific primers.
Descriptors		BLOOD TRANSFUSION GRANULOCYTES RECEPTORS, IGE NEUTROPENIA FLUORESCENT ANTIBODY TECHNIQUE AGGLUTINATION TESTS POLYMORPHISM (GENETICS)