Author/Editor		Škoberne, Mojca; Holtappels, Rafaela; Hof, Herbert; Geginat, Geernot
Title		Dynamic antigen presentation patterns of Listeria monocytogenes-derived CD8 T cell epitopes in vivo
Type		članek
Source		J Immunol
Vol. and No.		Letnik 167, št. 4
Publication year		2001
Volume		str. 2209-18
Language		eng
Abstract		Little information exists regarding the presentation of antigenic peptides in infected tissues. In this study the in vivo presentation of four different CD8 T cell epitopes of Listeria monocytogenes was monitored. Peptide presentation was measured by a new, highly sensitive, ex vivo Ag presentation assay that was based on the testing of freshly isolated cells from infected spleens with peptide-specific CD8 T cell lines in an IFN-gamma-specific ELISPOT assay. Remarkably, the peptide presentation pattern of splenocytes and that of macrophages purified from spleens of L. monocytogenes-infected mice were different from those of in vitro infected macrophage-like cell lines. The in vivo Ag presentation pattern of splenocytes also exhibited dynamic changes during the first 48 h of infection. In vivo peptide presentation at later time points postinfection was biased toward immunodominant CD8 T cell epitopes, while at an early time point, 6 h postinfection, subdominant and dominant CD8 T cell epitopes were presented with similar strength. In summary, our studies show that Ag presentation during an infection is a highly dynamic process that only can be fully appreciated by the study of cells infected in their physiological environment.
Descriptors		ANTIGEN PRESENTATION LISTERIA MONOCYTOGENES CD8-POSITIVE T-LYMPHOCYTES EPITOPES, T-LYMPHOCYTE MICE BACTERIAL PROTEINS ENZYME-LINKED IMMUNOSORBENT ASSAY HEAT-SHOCK PROTEINS KINETICS LYMPHOCYTE COUNT MACROPHAGES ORGAN SPECIFICITY PEPTIDE FRAGMENTS SPLEEN TUMOR CELLS, CULTURED