Author/Editor		Kožuh-Eržen, N
Title		Analitska metoda za ugotavljanje zaostankov klorpromazina v mišičnem tkivu in urinu klavnih živali
Translated title		Analytical procedure for the determination of chlorpromazine residues in muscle tissue and urine of food-producing animals
Type		članek
Source		Slov Vet Res
Vol. and No.		Letnik 38, št. 4
Publication year		2001
Volume		str. 305-12
Language		slo, eng
Abstract		Inspection of drug residues is of great importance in veterinary medicine. This is especially important in Slovene veterinary medicine where the use of certain drugs is prohibited for food-producing animals. This includes drugs containing chlorpromazine as an active compound. A highly sensitive and selective analytical method has been introduced for the determination of chlorpromazine residues in the muscle tissue and urine of food-producing animals. It includes an appropriate extraction and sample clean-up, using solid phase extraction, and is then followed by the determination using high-performance liquid chromatography (HPLC) with UV detection. In spite of the relatively low recovery of the method, it enables a low detection limit and good selectivity.
Summary		Nadzor zaostankov zdravil, posebno tistih, ki so v veterinarski medicini v Sloveniji prepovedana za uporabo pri živalih, namenjenih za prehrano ljudi, je izredno pomemben. Med taka spadajo tudi zdravila, ki kot aktivno učinkovino vsebujejo klorpromazin. V ta namen smo za ugotavljanje vsebnosti zaostankov klorpromazina v mišičnem tkivu in urinu živali, namenjenih za prehrano ljudi, uvedli dovolj občutljivo in selektivno analitsko metodo. Ta je vključevala v prvi fazi ekstrakcijo zaostankov klorpromazina iz vzorcev mišičnega tkiva in urina ter čiščenje ekstraktov z ekstrakcijo tekoče-trdno, v drugi fazi pa njihovo ugotavljanje s tekočinsko kromatografijo visoke ločljivosti (HPLC) z UV zaznavanjem. Kljub razmeroma slabemu izkoristku smo s to analitsko metodo dosegli dobro ponovljivost, nizko mejo zaznavnosti in zadovoljivo selektivnost.
Descriptors		MEAT FOOD ANALYSIS DRUG RESIDUES MUSCLE, SKELETAL CHLORPROMAZINE CHROMATOGRAPHY, HIGH PRESSURE LIQUID