Author/Editor     Logar, Dušan
Title     Povezava genov lokusa DQ glavnega histokompantibilnega kompleksa (MHC) s tvorbo avtoprotiteles proti antigenom Ro pri bolnikih s sistemskim lupusom eritematozusom
Type     monografija
Place     Ljubljana
Publisher     Medicinska fakulteta
Publication year     2001
Volume     str. 69
Language     slo
Abstract     Systemic lupus erythematosus (SLE) is a complex autoimmune disease due to the interplay of various genetic, hormonal and enviromental factors. Genetic factors predispose an individual not only to develop the SLE itself, but also to exibit various clinical and serological features of this disease. Among Caucasians, the associations with the extended haplotypes DRB1*03, DRB3*0101, DQB1*0501, DQB1*0201 and DRB1*1501, DQA1*0102, DQB1*0602 were recorded. The associations of specific HLA-class II alleles with the production of various autoantibodies in SLE is even stronger than with the disease itself. The combination of DQB1*0201 and one of DQA1*0101, DQA1*0102 or DQA1*0103 is associated with anti-Ro antibodies. Anti52-kDa Ro antibodies are linked to HLA-DRB1*0301, DQA1*0501, DQB1*0201. The purpose of this study was to investigate if the presence or absence of anti-Ro alone autoantibodies in SLE could be attributed to different antigen presenting capacity of HLA molecules coded by polymorphic HLA-DR or HLA-DQ genes. A cohort of 65 female SLE patients was included in the study. All patients were typed for HLA-DRB1, DQA1 and DQB1 alleles by polymerase chain reaction-based sequence specific oligonucleotide hybridisation (PCR-SSO) and by ampflification of particular alleles with sequence-specific primers (PCR-SSP). The control group consisted of 74 healthy Slovenians who were randomly chosen from the group typed for HLA-DRB1, DQA1 and DQB1 alleles by PCR-SSO and PCR-SSP. The presence of autoantibodies against Ro antigens was ascertained by counterimmunoelectrophoresis (CIE) and subsequently confirmed by enzyme-linked immunosorbent assay (ELISA). The prediction that some specific HLA-class II alleles were associated with SLE while the others HLA-class II alleles may protect an individual against this disease, was confirmed by our study. (Abstract trunacted at 2000 characters).
Descriptors     LUPUS ERYTHEMATOSUS, SYSTEMIC
AUTOANTIBODIES
GENES, MHC CLASS II
HLA-DR1 ANTIGEN
HLA-DQ ANTIGENS
HAPLOTYPES
ALLELES
HISTOCOMPATIBILITY TESTING
POLYMERASE CHAIN REACTION
COUNTERIMMUNOELECTROPHORESIS
ENZYME-LINKED IMMUNOSORBENT ASSAY