Author/Editor		Čemažar, Maja
Title		A comparison of gene delivery methods using electroporation and lipofectin-based techniques in vitro and in vivo
Type		članek
Source		In: Kotnik T, Miklavčič D, editors. MEDICON 2001: Mediterranean conference on medical and biological engineering and computing. Proceedings of the workshop on Electroporative assisted drug delivery: electrochemotherapy and genetherapy; 2001 Jun 11; Pula. Ljubljana: Faculty of electrical engineering,
Publication year		2001
Volume		str. 17-9
Language		eng
Abstract		We have compared electroporation, lipofectin and integrin-targeted lipofectin to deliver the marker gene green fluorescent protein (GFP) to cells in vitro and to tumours in vivo. In vitro experiments in rodent and human tumour cell lines showed a variation in transfection efficiency depending on the method and cell line. Integrin-mediated transfection and electroporation were superior with up to 66% and 57% of cells transfected, respectively. Our in vivo data showed that different tumours have different susceptibility to transfection. Electroporation showed a higher efficiency than lipofectin-based methods, with up to 6% of cells transfected in the tumours. Transfection efficiency was very heterogeneous and GFP fluorescence varied both qualitatively and quantitatively between tumour models and treatments. GFP expression in tumours growing in window chambers was observed already 5 h following transfection, reaching maximal value by 2 days and stayed at this level throughout the observation period.
Descriptors		ELECTROPORATION TRANSFECTION NEOPLASMS, EXPERIMENTAL TUMOR CELLS, CULTURED MICE RATS INTEGRINS GENE THERAPY