Author/Editor		Trček, Janja; Wilharm, Gottfried; Jacobi, Christoph A.; Heesemann, Juergen
Title		Yersinia enterocolitica YopQ: strain-dependent cytosolic accumulation and post-translational secretion
Type		članek
Source		Microbiology
Vol. and No.		Letnik 148
Publication year		2002
Volume		str. 1457-65
Language		eng
Abstract		YopQ in Yersinia enterocolitica (YopK in Yersinia pseudotuberculosis) is a type III secreted protein required for virulence of yersiniae. In this study YopQ expression, secretion and nucleotide sequences of the corresponding yopQ gene from different yersinia strains were analysed. The cytosolic accumulation differed significantly among serotypes of Y. enterocolitica. These differences might be attributable to variations in the nucleotide sequence and their consequences on mRNA secondary structure. An mRNA signal hypothesis has been proposed for YopQ, predicting the coupling of translation and secretion via an mRNA signal. This hypothesis claims a strictly co-translational secretion of YopQ without its intracellular accumulation. The presence of YopQ in the cytosol, even with a closed secretion apparatus, is demonstrated. Moreover, post-translational secretion of YopQ could be demonstrated. These findings do not support the mRNA signal hypothesis for co-translational secretion.
Descriptors		YERSINIA ENTEROCOLITICA YERSINIA PSEUDOTUBERCULOSIS VIRULENCE BACTERIAL PROTEINS CYTOSOL BASE SEQUENCE TRANSLATION, GENETIC FLOW CYTOMETRY BLOTTING, WESTERN