Author/Editor		Renaudineau, Y; Grunebaum, E; Krause, I; Praprotnik, Sonja; Revelen, R; Youinou, P; Blanks, M; Gilburd, B; Sherer, Y; Luderschmidt, C
Title		Anti-endothelial cell antibodies (AECA) in Systemic sclerosis - increased sensitivity using different endothelial cell substrates and association with other autoantibodies
Type		članek
Source		Autoimmunity
Vol. and No.		Letnik 33
Publication year		2001
Volume		str. 171-9
Language		eng
Abstract		Objective: One of the main features of systemic sclerosis (SSc) is vascular damage, the mechanism of which is not understood. In the present study we examined whether screening of SSc patients for different anti-endothelial cells antibodies (AECA) tit various origins increase the sensitivity of AECA detection in SSc patients. Secondary aim was an attempt to correlate AECA with other common auttiantib<xiies. Materials & metods: 478 SSc patients were studied for the presence AECA, anti-cardiolipin (aCL). anti-dsDIVA. anti-heparin (AHA), anti-pyruvate dehydrogenase (PDH) and anti-PDC-E2 autoantibodies. AECA levels were determined using human umbilical vein EC (HUVEC), bone marrow EC (BMEC). EC hybridoma (EA.hy 926) and Kaposi sarcoma EC (KS). Results: Positive AECA were found in 49.5% of SSc patients (37.19c HUVEC: 34.3% BMEC; 26.3% EaHy 926 and 22.7% KS). The highest percent reactivity of AECA was obtained using microvascular BMEC. When combining BMEC and either other cell lines the reactivity ranged from 41.4% to 46%. A signiticant association between AECA on the one hand and AHA (p<0.001) and anti-PDH (p<0.05) on the other was seen. Cross-reactivity with anti-PDC-E2 was excluded by inhibition tests. but AHA and anti-PDH may be part of the spectrum tit AECA. Conclusions: Since false-negative AECA may result from lack of expression of various antigens on a specitic EC. analysis of AECA in SSc patients requires using several EC types including microvascular EC.
Descriptors		SCLERODERMA, SYSTEMIC AUTOANTIBODIES ANTIBODIES, ANTICARDIOLIPIN ANTIBODIES, ANTINUCLEAR SENSITIVITY AND SPECIFICITY PYRUVATE DEHYDROGENASE COMPLEX HEPARIN ENZYME-LINKED IMMUNOSORBENT ASSAY ENDOTHELIUM CROSS REACTIONS CELL LINE