Author/Editor		Jurič, Damijana Mojca
Title		Vpliv interlevkina-1 in drugih citokinov na sproščanje živčnega rastnega dejavnika iz astrocitov možganske skorje podgane
Translated title		The effect of interleukin-1 and other cytokines on nerve growth factor release from rat cortical astrocytes
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		2002
Volume		str. 106
Language		slo
Abstract		Introduction. In the central nervous system, a neurotrophic compound nerve growth factor (NGF) is synthesised by neuronal and non-neuronal cells. Various pharmacological agents including growth factors, neurotransmitters and cytokines can modulate its synthesis and secretion. Interleukin-1beta (IL-1beta) is a potent and specific stimulator of NGF synthesis and secretion from astrocytes. The present study was aimed at identifying astroglially-localised IL-1 receptors, determining their pharmacological properties and testing the hypothesis that the action of cytokines of IL-1 family (IL-la, IL-1beta IL-lra) on NGF release from astrocytes is under the control of IL-1 receptors. In addition, the effects of other cytokines (TNF-a, IL-6, IL-18, TGF-beta1) in the regulation of basal and IL-beta-stimulated NGF secretion from astxocytes were examined. Methods. Primary cultures of astrocytes were prepared from cortices of newborn rats. The pharmacological properties of astroglially-localised interleukin-1 type I and type II receptors were determined by radioligand binding studies using (125I) IL-1beta (IL-1 receptor specific agonist). Enzyme-immuno assay was used to study the regulation of NGF secretion. Results. The results revealed the existence of a homogenous population of (lasl) IL-1beta binding sites with KD of 60.7 +- 7.4 pM and Bmax of 2.5 +- 0.1 finol mg 1 protein. IL-1 receptor agonists IL-1beta (K; = 59,9 pM) and IL-la (K; = 341,1 pM) as well as the antagonist IL-lra (K; = 257,5 pM), displaced specific (lasl) IL-1beta binding from cultured astrocytes in a monophasic manner. Anti-IL-1RI antibody completely blocked specific (i25l) IL-1beta binding (ICso = 6,3 ng/ml) while anti-IL-1RII antibody had no inhibitory effect. Using receptor-binding studies, the presence of IL-1 type I receptors on rat neonatal cortical astrocytes was confirmed. IL-1 type II receptors are not present on these cells. (Abstract truncated at 2000 characters)
Descriptors		ASTROCYTES NERVE GROWTH FACTORS INTERLEUKIN-1 RECEPTORS, INTERLEUKIN-1 INTERLEUKIN-6 RATS, WISTAR CELLS, CULTURED BRAIN CONCUSSION ANIMALS, NEWBORN ENZYME-LINKED IMMUNOSORBENT ASSAY RADIOLIGAND ASSAY TUMOR NECROSIS FACTOR TRANSFORMING GROWTH FACTOR BETA